Journal: International Journal of Molecular Sciences

Article Title: Development of Metal Complexes for Treatment of Coronaviruses

doi: 10.3390/ijms23126418

Figure Lengend Snippet: Auranofin inhibits SARS-CoV-2 proliferation in living organisms. Huh7 infection is associated with SARS-CoV-2 for 2 h at a multitude of infected (MOI) from one before ever being incubated with 4 M auranofin or 0.1 DMSO. Cell lysates and culturing filtrates were obtained 24 and 48 h after transmission, and viral RNA rates were measured using RT-PCR using primers and probe targeting the SARS-CoV-2 N1 and SARS-CoV-2 N2 regions. After measuring, standardizing, and correcting the human RNA extracted form tumor cells, the viral RNA values per ug of total cellular RNA were determined. The outcomes were compared for all specific primers, revealing a significant drop in viral RNA following 24 and 48 h. SARS-CoV-2 transmissibility titers were obtained using a cell test.48 h after invasion, in culturing filtrates The data are the mean SEM of two separate tests performed in duplicate, t -test ** p < 0.001. Reprinted/adapted with permission from Ref. .

Article Snippet: Most of the drugs studied had a good binding affinity to the SARS-CoV-2 major protease (M pro ), which was equivalent to Remdesivir, according to molecular docking calculations.

Techniques: Infection, Incubation, Transmission Assay, Reverse Transcription Polymerase Chain Reaction

Journal: International Journal of Molecular Sciences

Article Title: Development of Metal Complexes for Treatment of Coronaviruses

doi: 10.3390/ijms23126418

Figure Lengend Snippet: Auranofin-treated cells have a dose-dependent decrease in SARS-CoV-2 RNA: Auranofin (0.1–10 μM) was applied to SARSCOV-2 infected Huh7 cells in repeated dilutions. RT-PCR was used to quantify viral RNA in cell pellets and culture supernatants using primers and probes targeting the SARS-CoV-2 N1. The data were graphed using a non-linear regression model (GraphPad software). Auranofin reduced virus replication in infected cells with an EC50 of about 1.4 μM. The 50% cytotoxic concentration was around 5.7 μM. The data represent two separate tests that were carried out in duplicate, with permission from .

Article Snippet: Most of the drugs studied had a good binding affinity to the SARS-CoV-2 major protease (M pro ), which was equivalent to Remdesivir, according to molecular docking calculations.

Techniques: Infection, Reverse Transcription Polymerase Chain Reaction, Software, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Development of Metal Complexes for Treatment of Coronaviruses

doi: 10.3390/ijms23126418

Figure Lengend Snippet: Auranofin therapy significantly inhibits the incidence of SARS-CoV-2-induced immune cells in living organisms: qRT-PCR was used to determine mRNA levels of IL-6, IL-1, TNF, and NF-kB at 24 and 48 h after transmission. Following correcting the GAPDH gene, the fold variation in tumor cells compared to baseline controls was determined. The data shows the mean ± SEM of two separate tests that were performed in parallel. ** p < 0.001. Reprinted/adapted with permission from Ref. .

Article Snippet: Most of the drugs studied had a good binding affinity to the SARS-CoV-2 major protease (M pro ), which was equivalent to Remdesivir, according to molecular docking calculations.

Techniques: Quantitative RT-PCR, Transmission Assay

Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet: Neutralizing mAbs as pre-exposure prophylaxis in k18-hACE2 mice CV1-1, CV1-30, and CV2-75 were assessed to see whether they could confer protection in a mouse model. (A) Experimental timeline. (B) Number of PFUs in the lungs 2 days following challenge. Error bars indicate SD. (C) Viral RNA in lung tissue 2 days after challenge was measured by qPCR and normalized to GAPDH expression. Error bars indicate SD. (D) Kaplan-Meyer survival curve of the viral load/titer in the lungs of remaining mice comparing the various treatment groups. Statistics were determined by one-way ANOVA with Dunnett’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Expressing, Comparison

Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet:

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Virus, Infection, Recombinant, Affinity Column, Purification, Staining, Cloning, Cell Isolation, Transfection, Luciferase, Reverse Transcription, Gene Expression, Nested PCR, Sequencing, Expressing, Plasmid Preparation, Software, Microscopy